Furlanetto 2018 Physiol Plant
|Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Møller IM (2018) Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. Physiol Plant [Epub ahead of print].|
Abstract: We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high-temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6h, respectively, while 2h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. AP endonuclease, measured in extracts from cells incubated for 2h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30 min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited by 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high-temperature events associated with global warming.
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Labels: MiParea: Respiration
Stress:Temperature Organism: Plants
Preparation: Intact cells
Coupling state: ROUTINE, ET Pathway: N, CIV, ROX HRR: Oxygraph-2k