FN

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MitoPedia

FN

Description

F-junction

FN is induced in mt-preparations by addition of NADH-generating substrates (N-pathway control state, or CI-linked pathway control) in combination with one or several fatty acids, which are supplied to feed electrons into the F-junction through fatty acyl CoA dehydrogenase (reduced form FADH2), to electron transferring flavoprotein (CETF), and further through the Q-junction to Complex III (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting), but simultaneously generates FADH2 and NADH and thus depends on N-junction throughput. Hence FAO can be inhibited completely by inhibition of Complex I (CI). This physiological substrate combination is required for partial reconstitution of TCA cycle function and convergent electron-input into the Q-junction, to compensate for metabolite depletion into the incubation medium. FS in combination exerts an additive effect of convergent electron flow in most types of mitochondria.

Abbreviation: FN

Reference: Electron transfer-pathway state, Gnaiger 2014 MitoPathways


MitoPedia concepts: Respiratory state, SUIT state, Recommended 


MitoPedia topics: EAGLE 

Communicated by Gnaiger E, edited 2019-01-22 by Komlodi T.

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FNL

FN pathway in the LEAK state can be evaluated in the following SUIT protocols:

FNP

FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

FNE

FN pathway in the ET state can be evaluated in the following SUIT protocols: