Dechandt 2018 Am J Physiol Regul Integr Comp Physiol
|Dechandt CR, Vicentini TM, Lanfredi GP, da Silva RMP Jr, Espreafico EM, de Oliveira JAC, Faça VM, Garcia-Cairasco N, Alberici LC (2018) The highly efficient power house in the Wistar Audiogenic Rat (WAR), an epileptic rat strain. Am J Physiol Regul Integr Comp Physiol [Epub ahead of print].|
Abstract: The Wistar Audiogenic Rat (WAR) is an animal model of tonic-clonic epileptic seizures, developed after genetic selection by sister x brother inbreeding of Wistar rats susceptible to sound stimuli. Although metabolic changes have been described in this strain, nothing, however, is known about its mitochondrial metabolism. Here, we addressed mitochondrial aspects of OXPHOS, oxidative stress, biogenesis and dynamics in liver, skeletal muscle and heart of male WAR, correlating then to physiological aspects of body metabolism. The results showed higher mitochondrial content, respiration rates in phosphorylation and noncoupled states and H2O2 production in WAR. Liver presented higher content of PGC1α and mTOR, proteins related to mitochondrial biogenesis. In agreement, isolated liver mitochondria from WAR showed higher respiration rates in phosphorylation state and ADP/O, as well as higher content of proteins related to electron transport chain ATP synthase, Krebs cycle, mitochondrial fusion and fission. Mitochondria with higher area and perimeter, and more variable shapes were found in liver and soleus from WAR, in addition to lower GSH/GSSG. In vivo WAR demonstrated lower body mass and energy expenditure, but higher food and water intake and amino acid oxidation. When exposed to a running test, WAR reached higher speed and resisted for longer time and distance than their Wistar controls. In conclusion, WAR strain has mitochondrial changes in liver, skeletal muscle and heart which improves its mitochondrial capacity of ATP production, making it an excellent rat model to study PGC1α overexpression and mitochondrial function in different physiological conditions or facing pathological challenges.
• Keywords: Wistar audiogenic rat, Body metabolism, Experimental epilepsy model, Mitochondria, Reactive oxygen species • Bioblast editor: Plangger M
Labels: MiParea: Respiration, nDNA;cell genetics, Exercise physiology;nutrition;life style
Organism: Rat Tissue;cell: Heart, Skeletal muscle, Liver Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET Pathway: N, ROX HRR: Oxygraph-2k