Cruz 2018 Chem Biol Interact

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Cruz LC, Ecker A, Rodrigues NR, Martins IK, Posser T, Maciel FE, Vargas MA, Barbosa NV, Franco JL (2018) Honey protects against wings posture error and molecular changes related to mitochondrial pathways induced by hypoxia/reoxygenation in adult Drosophila melanogaster. Chem Biol Interact 291:245-52.

» PMID: 29964003

Cruz LC, Ecker A, Rodrigues NR, Martins IK, Posser T, Maciel FE, Vargas MA, Barbosa NV, Franco JL (2018) Chem Biol Interact

Abstract: We conducted an investigation to evaluate the effects of Brazilian Pampa biome honey and its major phenolic compounds on the development of an erected wings posture phenotype and related mitochondrial aspects induced by Hypoxia/Reoxygenation (H/R) in Drosophila melanogaster. Flies were pre-treated for 3 days with a 10% honey solution and different concentrations of caffeic acid and ρ-coumaric acid and then submitted to hypoxia for 3 h. We observed that after reoxygenation, some flies acquired an erected wings posture and that this feature may be related to mortality. In addition, H/R induced down-regulation of ewg mRNA expression, which could be associated to the observed complex phenotype. H/R also caused a dysregulation in opa1-like, ldh and diap genes expression and reduced O2 fluxes in flie's mitochondria. Honey mitigated opa1-like mRNA expression changes provoked by H/R. Differently from honey, caffeic and ρ-coumaric acids displayed no protective effects. In conclusion, we report for the first time the protective effects of honey against complex phenotypes and mitochondrial changes induced by H/R in adult flies.

Keywords: Ewg, Honey, Hypoxia, Mitochondria, Wing posture, opa1-like Bioblast editor: Plangger M, Kandolf G O2k-Network Lab: BR Sao Gabriel Franco JL


Labels: MiParea: Respiration, nDNA;cell genetics, Exercise physiology;nutrition;life style 

Stress:Hypoxia  Organism: Drosophila 

Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS  HRR: Oxygraph-2k 

Lables, 2018-08