Banerjee 2018 Stem Cells Int
|Banerjee A, Lindenmair A, Steinborn R, Dumitrescu SD, Hennerbichler S, Kozlov AV, Redl H, Wolbank S, Weidinger A (2018) Oxygen tension strongly influences metabolic parameters and the release of interleukine-6 of human amniotic mesenchymal stromal cells in vitro. Stem Cells Int 2018:9502451.|
Abstract: The human amniotic membrane (hAM) has been used for tissue regeneration for over a century. In vivo (in utero), cells of the hAM are exposed to low oxygen tension (1–4 % oxygen), while the hAM is usually cultured in atmospheric, meaning high oxygen tension (20 % oxygen). We tested the influence of oxygen tensions on mitochondrial and inflammatory parameters of human amniotic mesenchymal stromal cells (hAMSCs). Freshly isolated hAMSCs were incubated for 4 days at 5 % and 20 % oxygen. We found 20 % oxygen to strongly increase mitochondrial oxidative phosphorylation, especially in placental amniotic cells. Oxygen tension did not impact levels of reactive oxygen species (ROS), however, placental amniotic cells showed lower levels of ROS, independent of oxygen tension. In contrast, the release of nitric oxide was independent of the amniotic region, but dependent on oxygen tension. Furthermore, IL-6 was significantly increased at 20 % oxygen. To conclude, short time cultivation at 20 % oxygen of freshly isolated hAMSCs induced significant changes in mitochondrial function and release of IL-6. Depending on the therapeutic purpose, cultivation conditions of the cells should be chosen carefully for providing the best possible quality of cell therapy.
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET