Amytal

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MitoPedia

Amytal

Description

Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.

Abbreviation: Amy



MitoPedia topics: Inhibitor 

Application in HRR

Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25. The product is withdrawn from Sigma but some residual amounts are still available.

Caution: Light sensitive and toxic!


Preparation of 200 mM stock solution (dissolved in 99.9% ethanol):

1) Weigh 49.65 mg of amytal.
2) Dissolve in 10 ml 50% ethanol.
3) Divide into 500 µl aliquots (use dark glass vials - light sensitive).
4) Store at -20 °C.